Figure 7.

The Targeting of EGFP Fusion Proteins to the Chloroplast Envelope by the Reverse Sequences of Chloroplast Transit Peptides.

Details are the same as in Figure 4.

(A) The reverse sequence of Arabidopsis Rubisco small-subunit transit peptide fused to the C terminus of EGFP-Toc34G.

(B) The reverse sequence of B. sinuspersici pyruvate orthophosphate dikinase transit peptide fused to the C terminus of EGFP-Toc34G.

(C) The reverse sequence of B. sinuspersici ferredoxin transit peptide fused to the C terminus of EGFP-Toc34G.

(D) The reverse sequence of B. sinuspersici NAD-malic enzyme mitochondrial presequence fused to the C terminus of EGFP-Toc34G.

(E) The mitochondrial presequence of NAD-malic enzyme fused to the N terminus of EGFP.

(F) The mature protein of B. sinuspersici ferredoxin fused to the C terminus of EGFP.

(G) The reverse sequence of B. sinuspersici ferredoxin transit peptide fused to the C terminus of the fusion protein as shown in (F).

(H) The reverse sequence of the CT of B. sinuspersici Toc132 fused to the C terminus of EGFP.

(I) The reverse sequence of the CT of B. sinuspersici Toc159 fused to the C terminus of EGFP.

[See online article for color version of this figure.]