Table 2. ChloroP Prediction of Putative Chloroplast Transit Peptides.
| Protein | Orientation | cTP Scorea | cTP Length (Residues)b | Cleavage Site Scorec |
|---|---|---|---|---|
| B. sinuspersici Toc159-1 | Reverse | 0.519 | 51 | 10.81 |
| Other Toc159 (n = 32) | Reverse | 0.486 ± 0.024 | 48.3 ± 11.4 | 4.00 ± 3.30 |
| Arabidopsis Rubisco small subunit | Forward | 0.572 | 54 | 10.09 |
Prediction was performed using the ChloroP 1.1 server (Emanuelsson et al., 1999; http://www.cbs.dtu.dk/services/ChloroP).
a
A value of >0.5 predicts the presence of a transit peptide.
b
The length is defined based on the cleavage site prediction.
c
A more positive value indicates a higher probability of stromal processing peptidase cleavage.