Table 1. Steady State Kinetic Constants for the MKS1 Wild Type and G129W Tunnel-Blocking Variant.
Substrate | MKS1 |
G129W |
||||
---|---|---|---|---|---|---|
Kcat | Km | Kcat/Km | Kcat | Km | Kcat/Km | |
3-ketomyristate | 227.9 | 18.4 | 206,667 | 257.5 | 34.49 | 124,432 |
3-ketoheptanoate | – | >>1500 | 11 | 10.53 | 319.2 | 550 |
Values for Kcat, Km, and Kcat/Km are min−1, µM, and M−1⋅s−1, respectively.