Figure 11.

Single Amino Acid Substitutions within the External Crevice of Arabidopsis mBIO3-BIO1 Affect Substrate Channeling.

(A) Close-up view of the crevice of the wild-type (WT) and mutant enzymes. The crevice is shown with an arrow. Tyr-360 and Trp-793 are colored in blue and pink, respectively.

(B) Kinetic study of mBIO3-BIO1 (wild-type, S360Y, and I793W variants) overall catalyzed reaction. DAPA-AT + DTBS activity (time-course formation of DTB) was assayed as in Figure 4A. Data are from a representative experiment repeated three times.