Figure 5.

RNAPII CTD S5-Phosphorylation Is Required for 5′-Capping of miRNA and Ta-siRNA Precursors.

For detection and amplification of uncapped miRNA precursors, a T4 RNA ligase–mediated isolation procedure (Jiao et al., 2008) was used with RNA samples of three independent biological replicates.

(A) Accumulation of uncapped small RNA precursors in the d123* mutant at 14 DAG.

(B) Comparison of mature small RNA levels in wild-type and d123* mutant seedlings at 14 DAG. Normalization of total RNAs was used to ensure equal loading.