Table 1. qRT-PCR Analysis of Small RNA Precursor and Protein Coding Transcripts, Which Show Reduced Levels in the cdkf;1 Mutant Compared to the Wild Type at 7 DAG in the Total RNA and Poly(A+) RNA Fractions.
Gene | Poly(A)− and Poly(A)+ | Poly(A)+ | |||
---|---|---|---|---|---|
Arabidopsis Genome Initiative | Wild Type/cdkf;1 | sd | Wild Type/cdkf;1 | sd | |
DCL1 | At1G01040 | 1.36 | 0.18 | 1.93 | 0.11 |
HYL1 | At1G09700 | 1.18 | 0.21 | 1.74 | 0.07 |
HST1 | At3G05040 | 1.32 | 0.10 | 2.10 | 0.19 |
AGO7 | At1g69440 | 2.17 | 0.12 | 3.18 | 0.30 |
MIR156 h | AT5G55835 | 1.70 | 0.08 | 2.85 | 0.17 |
MIR157c | AT3G18217 | 1.36 | 0.13 | 2.82 | 0.35 |
MIR168b | AT5G45307 | 1.99 | 0.28 | 2.07 | 0.08 |
MIR169f | AT3G14385 | 2.51 | 0.33 | 5.46 | 0.29 |
MIR172b | At5g04275 | 5.47 | 0.63 | 24.68 | 2.34 |
MIR319a | At4G23713 | 2.58 | 0.31 | 4.00 | 0.20 |
TAS2 | At2g39681 | 1.02 | 0.09 | 2.12 | 0.21 |
TAS3 | At3G17185 | 2.68 | 0.18 | 2.4 | 0.31 |