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. 2012 Apr 6;24(4):1654–1674. doi: 10.1105/tpc.112.096768

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 7. — GTPase Activity of the Nucleoid Localizing NOA1 Correlates with NO Accumulation.

(A) Percentage nucleoids showing fluorescence in water-, glycerol-, or pathogen-treated plants. Nucleoids were purified from treated plants and assayed for fluorescence under a confocal microscope. The experiment was repeated twice with similar results.

(B) GTPase activity associated with nucleoids purified from water-, glycerol-, or pathogen-treated plants at 12 h after inoculation. Protein was extracted from 10⁸ nucleoids, and E. coli–purified NOA1 protein was used as a positive control. The experiment was repeated twice with similar results.