Figure 4.

Localization and Quantification of H3K27me3 in 2i and Serum

(A) Binning of H3K27me3 -enriched regions in 2i and serum according to tag densities per peak (in reads/kb). The calculated genomic background is 5 reads/kb per 10 million mapped sequence reads (see Experimental Procedures).

(B) Genomic distribution of H3K27me3 peaks in 2i (left) or serum (right) per bin as shown in (A).

(C) Percentage of H3K27me3 reads present in the major repeat categories.

(D) Immunoblot analysis of histone modifications in total cell extracts.

See also Figure S5.