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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Mar 23;1818(10):2413–2423. doi: 10.1016/j.bbamem.2012.03.014

Figure 5. Treatment of human lymphocytes with rotenone induces monohydroxy and monoperoxy modifications of mitochondrial CL.

Figure 5

5A: Human lymphocyte contained tetralinoleoyl CL (m/z 1448) as its major CL species which eluted at 6.45 min in the second dimension system (C8 column, 100% sovent B). Lymphocytes treated with 250 μM rotenone (Ro) for 18h also displayed oxidized species eluting between 4-6 min. 5B: Non-oxidized CL spectrum from lymphocytes. 5C: Spectrum of CL-ox from lymphocytes treated with rotenone. Monoperoxy (m/z 1464) and hydroperoxy (m/z 1480) were the dominant oxidized CL species. Elemental compositions for CL masses are as follows: 1421.9443, C79H139O17P2; 1447.9654, C81H141O17P2; 1464.9632, C81H141O18P2; 1479.9638, C81H141O19P2.