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. Author manuscript; available in PMC: 2012 Jul 17.
Published in final edited form as: J Immunol. 2010 Nov 3;185(11):6728–6733. doi: 10.4049/jimmunol.1002543

FIGURE 5.

FIGURE 5

DRB treatment does not prevent NA maturation or degradation. A, Western blot samples were prepared from DRB-treated, PR8-infected cells in the absence of DTT, along with lysates of untreated PR8-infected cells diluted as indicated. NA dimer (~98 kDa) and low levels of NA monomer (~50 kDa) were detected by Western blot. B, The NA signal from diluted PR8 samples was plotted, and the line of best fit was calculated and used to accurately measure levels of NA in DRB-treated cells. C, L-Kb cells were infected with PR8 and cultured for 6 h. After 1 h, cells were left untreated, or DRB was added. At 2 h p.i, cells were treated with MG132. Total-cell lysates were prepared and analyzed for NA expression by Western blot analysis, as in Fig. 1. Approximate molecular mass (in kDa) are shown.