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. 2010 Aug 27;101(12):2629–2636. doi: 10.1111/j.1349-7006.2010.01713.x

Figure 5.

Figure 5

 Activation and inhibition of epidermal growth factor receptor (EGFR) and Akt by epoxyeicosatrienoic acid (EET) and EET antagonists. (A) Effects of 11,12‐EET (1.0 μmol/L) on phosphorylated EGFR (p‐EGFR) (Tyr) in PC‐3 cells as determined by Bio‐Plex Phospho‐EGFR (Tyr) Assay Kit and normalized to total EGFR (t‐EGFR) (by western blot) at different times and control cells at 1 min and 120 min (n = 3). (B) Effects of 11,12‐EET (1.0 μmol/L), 14,15‐epoxyeicosa‐5(Z)‐enoic acid (14,15‐EEZE) (5.0 μmol/L), and a combination of 11,12‐EET and 14,15‐EEZE on p‐EGFR (Tyr) at 5 min treatment and normalized to the control (n = 3). *Significantly higher than control with P < 0.01. %Significantly lower than the control with P < 0.01. #Significantly lower than 11,12‐EET‐activated EGFR with P < 0.01. (C) Example of the effects of 11,12‐EET (1.0 μmol/L) on p‐Akt (Ser473) in PC‐3 cells at different times. Below the figure is the average of the p‐Akt band intensity to t‐Akt at each time point (n = 4). (D) Example of the effects of 11,12‐EET (1.0 μmol/L), 14,15‐EEZE (5.0 μmol/L), and a combination of 11,12‐EET and 14,15‐EEZE on p‐Akt (Ser473) at 60 min treatment. Below the figure is the average of the relative (p‐Akt/t‐Akt) in PC‐3 cells treated with 11,12‐EET and 14,15‐EEZE as normalized to the control cells (n = 4). *Significantly higher than control with P < 0.01. %Significantly lower than the control with P < 0.05. #Significantly lower than 11,12‐EET‐activated Akt with P < 0.01.