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In situ detection of phosphatidylinositol-3-kinase pathway activity in lipid rafts with the FRET reporter PARE. A schematic diagram of the PARE kinase activity reporter fused with the PDK-1 sequence. The FRET-based PDK-1 activation reporter, PARE, serves as a sensor for the phosphatidylinositol-3-kinase (PI3K), PDK-1 activation in lipid rafts. These upstream elements activate protein kinase B/Akt in a variety of cell types.
