Figure 5. A125 and D1-1 temperature, pH and Ca²⁺ dependencies.

A pH-dependent activity profiles for A125 and D1-1 at 40°C. B Temperature-dependency profiles for A125 and D1-1 activities. Activities were measured using 100 mM citric acid–Na₂HPO₄ (pH 4.0) and 100 mM Tris–HCl (pH 9.0) for A125 and D1-1, respectively. C Ca²⁺-dependency profiles for A125 and D1-1. Each value is the mean ± standard deviation (n = 3).