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. 2012 Jul 17;7(7):e41009. doi: 10.1371/journal.pone.0041009

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Malin E. V. Johansson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Muc2 (green antibody staining) labeled with GalNAz was detected by TAMRA (red). Within the first 5 h GalNAz-labeled mucin secretion only occurred from the surface epithelium or from cells in the very top of the crypts. Rarely, secretion of labeled mucin was observed after 6 h from goblet cells located in the upper part of the crypt (arrow). In the 8 h samples, TAMRA-staining detected GalNAz-labeled mucin in secreting crypt goblet cells and within the secreted mucus. Scale bars are 10 µm.