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Amplification yields of brain-derived (A, C, E, D) and PMCAb-derived (B, D, F, H) PrPSc for HY (A, B), 263K (C, D), SSLOW (E, F) and LOTSS (G, H) measured at 48 cycles. Three independent experiments for each condition were performed and average ± SD is shown. Statistical analyses were performed using Student's t-test. *P<0.05; **P<0.01; ***P<0.001; NS, non-significant.
