Figure 5. Analysis of growth rate of PMCAb-derived PrP^Sc.

PrP^Sc amplification yield plotted as a function of PMCAb cycle number (left panels) or cumulative incubation interval length (right panels) obtained in PMCAb reactions consisting of incubation intervals with lengths of 30 min (▴), 10 min (□) or 5 min (•) between sonications and seeded with PMCAb-derived HY (A, B), 263K (C, D), SSLOW (E, F) or LOTSS (G, H). Three independent experiments for each condition were performed and average ± SD is shown. To produce PMCAb-derived PrP^Sc, sPMCAb were seeded with brain-derived PrP^Sc, then six standard sPMCAb rounds were conducted.