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. 2012 Jul 17;7(7):e40813. doi: 10.1371/journal.pone.0040813

Figure 5. Time courses of the changes in PI fluorescence after NMDA treatment.

Figure 5

A: Representative confocal images of double-labeled immunofluorescent staining for Iba1 (microglial marker, green) and NeuN (neuronal marker, red) of vehicle-pretreated (control) and clodronate-pretreated slice cultures at 10 DIV. The number of Iba1-immunopositive microglia in clodronate-pretreated cultures was markedly decreased compared with that in control cultures. Scale bars = 100 µm. B, C: Representative images of PI fluorescence at 1, 3 and 5 days after NMDA treatment in control (B) and clodronate-treated (C) slices. Scale bar = 500 µm. D: PI positive areas were measured daily for 6 days after NMDA treatment in control (open circle) or clodronate-treated (closed square) slices. Data are expressed as a ratio to the positive area at day 1. Data are expressed as the mean ± SEM from eleven control or nine clodronate-treated slices. ***P<0.001 versus control slices (two-way ANOVA, followed by Bonferroni post hoc test).