Figure 7. Effects of MAP kinase inhibitors on PI fluorescence and microglial accumulation after NMDA treatment.

MAP kinase inhibitors were added to culture medium 1 day after NMDA treatment. A: Effects of MAP kinase inhibitors (10 µM each) on the attenuation of PI fluorescence after NMDA treatment. PI-positive areas were measured daily for 6 days after NMDA treatment in the presence of SB203580 (circle), SP600125 (triangle), PD98059 (diamond), or vehicle (square). Data are expressed as a ratio to the positive area at day 1. Data are expressed as the mean ± SEM from six to seven slices. **P<0.01, ***P<0.001 versus control slices (two-way ANOVA, followed by Bonferroni post hoc test). B: Concentration dependence of the suppressive effect of SB203580 on the attenuation of PI fluorescence after NMDA treatment. SB203580 at concentrations of 1 µM (closed triangle), 3 µM (closed diamond), and 10 µM (closed circle) were used. The open circle indicates the vehicle-treated group (control). Data are expressed as a ratio to the positive area at day 1. Data are expressed as the mean ± SEM from seven to eight slices. ^$ P<0.05 between control and 1 µM SB203580, ^# P<0.05, ^## P<0.01 between control and 3 µM SB203580, **P<0.01, ***P<0.001 between control and 10 µM SB203580 (two-way ANOVA, followed by Bonferroni post hoc test). C: Microglial accumulation in the injured areas 6 days after NMDA treatment in the presence of MAP kinase inhibitors (10 µM each) was quantitatively analyzed by calculating the P/R ratio. SB, SB203580; SP, SP600125; PD, PD98059. Data are expressed as the mean ± SEM from three slices.