Figure 4. DSP-PP₂₄₀ is cleaved by an activity secreted into conditioned medium by uninfected Sf9 cells.

CM_3–7d from virus infected cells (containing most intact DSP-PP₂₄₀), was mixed with an equal volume of 3d conditioned medium from uninfected Sf9 cells or with an equal volume of unconditioned Grace’s medium as described in Materials and Methods. At the times shown, medium samples were processed for native PAGE and Stains-All staining as described in Materials and Medthods. Percent processing of DSP-PP₂₄₀ was determined as described in Materials and Methods. Error bars represent standard deviation of at least duplicate samples.