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. 2012 Jul 17;7(7):e41110. doi: 10.1371/journal.pone.0041110

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Ritchie et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Stains-All staining of DSP-PP₂₄₀ cleavage. Lane 1: CM_0–3d of virus-infected cells without further incubation. Lane 2: CM_0–3d from virus-infected cells incubated for an additional 3days. Lane 3: Same reaction as lane 2 except with addition of EGTA (22 mM). Lane 4: Same reaction as lane 2 except with addition of 1mM 1,10-phenanthroline. Lane 5: Same reaction as lane 2 except with addition of 1 mM 1,10-phenanthroline and 1 mM ZnCl₂. M represents size marker. (B) Quantification of DSP-PP₂₄₀ processing shown in panel A and two similar gels. Numbers 1–5 correspond to lanes 1–5. Error bars represent standard deviation of the mean.