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. 2012 Jul 17;7(7):e41132. doi: 10.1371/journal.pone.0041132

Figure 1. Targeting of the mouse Neu3 gene.

Figure 1

A. Targeting strategy. Exons and introns are indicated by boxes and lines, respectively. Open boxes and filled boxes indicate non-coding and coding exons, respectively. Expression cassettes for the PGK-driven Neo gene (PGKNeobpA) and for diphtheria toxin A (DT-A) are indicated by thick gray boxes. The site of the probe for Southern blotting is indicated by a small open box. The sites of PCR primers are indicated by open arrows. Bg: BglII, RI: EcoRI, SS: Sse8387I. B. Southern blotting analysis of mice generated by crossing Neu3 heterozygous mice. Genomic DNA was prepared from a tail chip, digested by EcoRI, and analyzed by Southern blotting using the probe indicated in A. DNA fragments from the wild (W) and targeted (T) allele are indicated by arrows. C. PCR analysis of the homologous recombination in Neu3-deficient mice. Amplified DNA fragments from the wild (W) and targeted (T) allele are indicated by arrows.