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. 2012 Jul 17;10(7):e1001361. doi: 10.1371/journal.pbio.1001361

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Duchesne et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Average mean square displacement (MSD) as a function of time (mean ± sem) showing the different diffusion modes of diffusion of FGF2-NP (22 pM) in the pericellular matrix of living and of fixed Rama 27 cells. The number of subtrajectories corresponding to each mode of diffusion is indicated in parentheses. As not all subtrajectories lasted the duration shown on the graph, the minimal number (at late time) and the maximal number (early time) of subtrajectories are given (minimal-maximal). A similarly colour-coded trajectory is shown in the insert; scale bar, 1 µm. (B) MSD versus time interval (mean ± sem) for the confined diffusion modes of FGF2-NP (22 pM and 220 pM) in the pericellular matrix of living and fixed Rama 27 cells. The number of subtrajectories analysed are shown in parentheses. As not all subtrajectories lasted the duration shown on the graph, the minimal number (at late time) and the maximal number (at early time) of subtrajectories are given (minimal-maximal). NP embedded in PVA (polyvinyl alcohol) corresponds to isolated NPs embedded in thin film of polyvinyl alcohol on the surface of a glass coverslip, so are immobilized nanoparticles, and measures the inherent noise of the tracker. For Group 2, average MSD as a function of time data were fitted according to Equation (4), given in Materials and Methods. For clarity a quarter of the data points are shown in the graph. (C) Zoom in of (B) showing the MSD over time interval (mean ± sem) before confinement arises. (D) Calculated instantaneous coefficient of diffusion (Dins) according to Equation (2), given in Materials and Methods. (E) Calculated diameter of confinement (dconf). The p values according to the Kolmogorov-Smirnov non-parametric test are given in Table S3.