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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Blood Cells Mol Dis. 2012 May 25;49(2):102–106. doi: 10.1016/j.bcmd.2012.05.003

Figure 2. In vitro mouse bone marrow progenitor cell colony-forming and replating assays with HOXA9, HOXA9T and/or MEIS1.

Figure 2

Colony-forming and replating assays of mouse normal bone marrow progenitor cells transduced with MSCV-PIG+MSCVneo (i.e. control), HOXA9T_MSCV-PIG+MSCVneo (i.e. HOXA9T), HOXA9_MSCV-PIG+MSCVneo (i.e. HOXA9), MSCV-PIG+MEIS1_MSCVneo (i.e. MEIS1), HOXA9T_MSCV-PIG+ MEIS1_MSCVneo (i.e. HOXA9T+MEIS1) or HOXA9_MSCV-PIG+ MEIS1_MSCVneo (i.e. HOXA9+MEIS1). Duplicates were plated for each combination with 1×104 cells per dish, and every 7 days the cells were replated for up to three passages. Two independent experiments were conducted. Mean±SD values are shown. *, P<0.05, two-tailed t-test. (A) Average numbers of colonies per dish (≥ 50 cells/colony; 1×104 input cells) standard deviation of different passages (I, II and III) are shown. (B) Average cell numbers per dish and standard deviation values are shown.