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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Cancer Res. 2012 May 18;72(14):3677–3686. doi: 10.1158/0008-5472.CAN-12-0080

Figure 4.

Figure 4

POX contributes to the survival of cancer cells in response to hypoxia and glucose deprivation. HT29 cells were treated with low glucose (1mM) and/or hypoxia (0.05% O2) for 48 hrs. 10mM Dehydroproline (DHP), the POX inhibitor, and/or 5mM proline were given to the cells at the same time as indicated. A, proliferation assay was performed using the WST method. Intracellular ROS (B) and ATP production (C) were performed using the DCF assay and luciferase-based assay, respectively. D to F, POX siRNA (siPOX) was used to knock down POX expression when cells were treated with low glucose and/or hypoxia. Proliferation, ROS, and ATP production were detected as described as above.