Figure 5.

Protein expression patterns in vivo are consistent with findings in cultured HMEC strains. Immunofluorescence images of normal mammary glands from six individuals from two age groups: (A and B) are stained to show K14 (red) and K19 (green) expression, (C and D) are stained to show K14 (red) and K8 (green) expression; DAPI nuclear stain shown in all images (blue). Scale bar represents 100μm. Insets show a higher magnification view of the indicated locations within each image, the arrows in ‘B’ point to two clusters of K14⁺/K19⁺ cells. (E) 4μm serial sections shows a lobule from representative 37y and 76y women immunohistochemically stained to show expression of K19, cKit/CD117, smooth muscle actin (SMA), and K5/6 (brown), nuclei are stained blue. Scale bar represents 50μm.