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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Cell Immunol. 2012 May 19;276(1-2):26–34. doi: 10.1016/j.cellimm.2012.05.007

Figure 4. ZAP-70 is excluded from the immune synapses of SEB-mediated memory cell conjugates.

Figure 4

Conjugates were formed between DO11.10 naive and memory CD4 T cells, and APCs pulsed with SEB. At the indicated times, the conjugates were assessed for the presence of the TCR, ZAP-70 and pZAP-70. The two far-right columns in both panels show fluorescence overlays and the DIC images of the conjugates, respectively. Beneath the x-y images is a three-dimensional reconstruction along the x-z axis of a mature immune synapse. Data are representative of 3 separate experiments, examining a minimum of 50 conjugates per experiment.