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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Cell Immunol. 2012 May 19;276(1-2):26–34. doi: 10.1016/j.cellimm.2012.05.007

Figure 6. ZAP-70 migration to the immune synapse of SEB-mediated memory cell conjugates is regulated by fyn.

Figure 6

Conjugates were formed between DO11.10 × Fyn−/− memory CD4 T cells and APCs pulsed with SEB. At the indicated times, the conjugates were assessed for the presence of the TCR, ZAP-70 and pZAP-70. The two far-right columns show fluorescence overlays and the DIC images of the conjugates, respectively. Beneath the x-y images is a three-dimensional reconstruction along the x-z axis of a mature SEB-mediated immune synapse from naive or memory CD4 T cells. Data are representative of 3 separate experiments, examining a minimum of 50 conjugates per experiment.