Conjugates were formed between DO11.10 naive and memory CD4 T cells and APCs pulsed with SEB. After the 30 min (naive) or 5 min (memory), the mature synapses in the conjugates were assessed for the presence of the TCR, Fyn, and c-Cbl. The two far-right columns show fluorescence overlays and the DIC images of the conjugates, respectively. Data are representative of 2 separate experiments, examining a minimum of 50 conjugates per experiment.