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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Cell Immunol. 2012 May 19;276(1-2):26–34. doi: 10.1016/j.cellimm.2012.05.007

Figure 9. Fyn controls ZAP-70 recruitment to membrane lipid rafts of SEB-treated memory CD4 T cells.

Figure 9

DO11.10 × Fyn−/− naive and memory CD4 T cells were labeled with rhodamine-CTX-B (GM1) and were analyzed before or after conjugation with SEB-pulsed APCs. The cells either were fixed and then permeabilized for staining (control) or permeabilized with 1% Triton X-100, to solubilize and remove non-raft proteins. The conjugates were assessed to determine the presence of the TCR and ZAP-70. The two far-right columns in both panels show fluorescence overlays and the DIC images of the conjugates, respectively. Data are representative of 2 experiments, examining a minimum of 50 cells or conjugates per experiment.