Table I.

Percentages of Monomer, Dimer and High-Molecular-Weight Oligomers (HMWO) for the Insulin Oxidized Under Different Conditions and Native Insulin

Sample	%Monomer	%Dimer	%HMWO
Oxidized insulin in PB, pH 7.4	74.4 ± 0.9	18.8 ± 1.4	6.8 ± 1.2
Oxidized insulin in ABI, pH 8.0	96.1 ± 0.7	3.9 ± 0.7	NDa
Oxidized insulin in sodium citrate, pH 3	99.2 ± 0.3	0.8 ± 0.3	ND
Oxidized insulin in sodium citrate, pH 3.0, spiked into PB, pH 7.4	88.8 ± 1.3	9.3 ± 1.1	1.9 ± 0.1
Native insulin in PB, pH 7.4	99.4 ± 0.2	0.6 ± 0.2	ND

Based on SEC analysis with UV detection at 276 nm; data represent mean ± standard deviation of 3 individual batches

^aND = not detected