. 2012 May 10;29(8):2276–2293. doi: 10.1007/s11095-012-0755-z

Table II.

Fragments Identified in Reduced, Alkylated, ABS-Derivatized and Digested (Glu-C) Insulin and Oxidized Insulin, as Determined by Mass Spectrometry

Samples	Glu-C fragments oxidized	Glu-C fragments ABS-derivatized	Fragments oxidized	Fragments ABS-derivatized	Fragments Non-oxidized
Oxidized insulin	R_B22GFF^aY^aTPKT_B30 Fig. S1 A, B, C	R_B22GFFY^cTPKT_B30 Fig. S5	F^a _B1VNQHLCG_B8 Fig. S7	A_B14LY^cLVC_B19Fig. S10	L_A13YQLE_A17 Fig. S13
	A _B14LY^aLVCGE_B21 Fig. S2	R_B22GFFY^dTPKT_B30 Fig. S6	E_B21RGF^bFYTPKT_B30 Fig. S8	S_A12LY^cQLE_A17 Fig. S11	I_A10CSLY_A14 Fig. S14
	F^a _B1VNQHLCGSHLVE_B13 Fig. S3		F^a _B1VNQHLCGSHLVEAL_B15 Fig. S9	S_A12LY^dQLE_A17 Fig. S12	S_A12LYQLE_A17 Fig. S15
	F^b _B1VNQHLCGSHLVEALYLVCGE_B21 Fig. S4		H_B5LCGSH^aLVEAL_B15 MS/MS not shown		S_A12LYQL_A16 Fig. S16
			F_B1VNQH^aLCGS_B9 MS/MS not shown		Y_A14QLE_A17 Fig. S17
			F_B1VNQH^aLCGSHL_B11 MS/MS not shown		G_B8SHLVE_B13 Fig. S18
					H_B10LVEAL_B15 Fig. S19
					F_B1VNQHLCGS_B9 Fig. S20
					Y_B16LVC_B19 Fig. S21
					G_B20ERGFFY_B26 Fig. S22
					G_A1IVEQC_A6 Fig. S23
					A_B14LYLVCG_B20 Fig. S24
					Q_A15LENYCN_A21 Fig. S25
					L_B6CGSHLVE_B13 Fig. S26
Insulin	R_B22GFF^aY^aTPKT_B30 Fig. S1 A, B, C				G_B23FFYTPKT_B30 Fig. S27
Insulin					L_A13YQLE_A17 Fig. S13

oxidation of insulin was performed by MCO for 3 h at room temperature in 50 mM PB, pH 7.4

^aincorporation of one oxygen atom

^bincorporation of two oxygen atoms

^cincorporation of one ABS molecule

^dincorporation of two ABS molecules