Table 1.
Polymerase chain reaction (PCR) conditions for the 10 microsatellite loci used in the study of black bears fine-scale genetic structure, along with postamplification mix sets when ran on a 3100 ABI sequencer. T° C indicates the optimal annealing temperature.
| Locus | Fluorescent dye labeling | T°C | MgCl2 (mM) | Primers (μM) | Taq (units) | Post-PCR mix |
|---|---|---|---|---|---|---|
| G1D | FAM | 56.0 | 1.9 | 0.2 | 1.0 | mix 1 |
| G10H | FAM | 59.0 | 1.9 | 0.2 | 1.0 | mix 1 |
| G10L | NED | 56.0 | 1.9 | 0.3 | 2.0 | mix 2 |
| G10M | HEX | 62.0 | 1.6 | 0.3 | 0.6 | mix 4 |
| G10P | FAM | 58.0 | 1.5 | 0.4 | 1.3 | mix 3 |
| MU09 | NED | 60.0 | 1.0 | 0.3 | 1.5 | mix 1 |
| MU10 | HEX | 59.0 | 1.2 | 0.3 | 2.5 | mix 4 |
| MU15 | FAM | 56.0 | 0.8 | 0.3 | 1.6 | mix 1 |
| MU23 | HEX | 55.0 | 1.2 | 0.5 | 2.0 | mix 3 |
| MU50 | HEX | 55.0 | 2.0 | 0.5 | 1.6 | mix 2 |