Figure 1.

Variation of mitochondrial membrane potential during cell cycle progression. (A) Cell cycle distribution as assessed by propidium iodide (PI) fluorescence of Jurkat cells sorted initially for low (ΔΨ_mL) or high (ΔΨ_mH) mitochondrial membrane potential using TMRM. (B) Exponentially growing cultures from three different established cell lines were sorted based on TMRM fluorescence. Shown is the percentage of the sorted ΔΨ_mL (shaded bars) or ΔΨ_mH (open bars) cells that were in the G₁ phase. (C) Sorting strategy for G₁ cells with low and high ΔΨ_m. G₁ purified cells denoted in red, were subsequently analyzed for mitochondrial membrane potential as assessed by TMRM fluorescence. (D) Cell cycle analysis of G₁ cells sorted for low and high ΔΨ_m with quantitative post-sort purity derived from four independent experiments (mean ± SD). (E) Post-sort analysis of ΔΨ_m of G₁ cells sorted for low and high membrane potential compared to unsorted G₁ cells (shaded area).