Figure 4.

Hypoxia pushes stem cells to a neuronal fate, whereas enrichment increases the total stem cell pool. A–T, Representative Sox2/GFAP/eGFP immunostaining for all four groups; note the increase in number of Sox2+ cells expressing GFAP with environmental enrichment. U, Environmental enrichment also increased the number of reporter+/Sox2+ cells in both normoxic and hypoxic reared mice. V, Proportion of reporter+ cells that attain an immature neuronal (DCX+) or stem cell (Sox2+) fate is shown across groups. W, The percentage of Sox2+ stem cells that were committed to a neuronal lineage (colocalized with DCX) increased with hypoxic rearing, and this effect was attenuated with enrichment. X, The percentage of Sox2+ stem cells that remained in the NSC lineage (colocalized with GFAP) increased with enrichment. Y, Schematic representation of cell lineages arising from fate mapped GFAP+ astroglia. * denote significant increases from NSE; ** denotes significant increase from both NSE and HSE groups; — denotes significance from all other groups.