Table 1.
Primary antibodies
| Antibody | Manufacturer | Species | Dilution |
|---|---|---|---|
| β-Galactosidase | Cappel | Rabbit | 1:10,000 |
| β-Galactosidase | Abcam | Chicken | 1:500 |
| BrdU/CldU | Accurate Chemical | Rat | 1:250 |
| BrdU/IdU | Becton-Dickenson | Mouse | 1:100 |
| Doublecortin | Abcam | Rabbit | 1:2000 |
| Doublecortin | Santa Cruz | Goat | 1:500 |
| GFAP | Sigma | Mouse | 1:500 |
| GFAP | Dako | Rabbit | 1:1000 |
| GFP | Molecular Probes | Rabbit | 1:500 |
| GFP | Abcam | Chicken | 1:1000 |
| Ki67 | Vector Laboratories | Rabbit | 1:500 |
| Neuronal Nuclei | Chemicon | Mouse | 1:500 |
| Ng2 | Millipore | Mouse | 1:500 |
| Olig2 | Kindly provided by Drs. Charles Stiles and John Alberta, Dana-Farber Cancer Institute, Boston, MA | Rabbit | 1:20,000 |
| Sox2 | Chemicon | Goat | 1:200 |
| Ng2 | Chemicon | Rabbit | 1:500 |
| Sox2 | Chemicon | Goat | 1:200 |