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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: Genesis. 2010 Mar;48(3):183–194. doi: 10.1002/dvg.20604

Table 1.

Mouse Transgenic WNT Reporters

Driving the expression of a LacZ reporter
    TOPGal Three LEF/TCF consensus binding sites based on the pTOPFLASH reporter (Molenaar et al., 1996) with a c-fos minimal promoter (DasGupta and Fuchs, 1999)
    Axin2LacZ A knock-in of the LacZ gene with a nuclear localization signal in the Axin2 gene locus (Lustig et al., 2002)
    BAT-gal Seven TCF/LEF binding sites upstream of a 0.13-kb fragment containing the TATA box of siamois (Maretto et al., 2003)
    TCF/LEF-lacZ Six TCF/LEF response elements with an hsp68 minimal promoter (Mohamed et al., 2004)
    BATlacZ A TCF/LEF binding sequence upstream of a 0.13 kb Xenopus siamois minimal promoter; nuclear LacZ (Nakaya et al., 2005)
    ins-TOPGAL Six LEF/TCF consensus binding sites and a minimal thymidine kinase promoter derived from pTOPFLASH; nuclear LacZ (Moriyama et al., 2007)
Driving the expression of an EGFP reporter
    Ax2/d2EGFP A 5.6-kb DNA fragment including the Axin2 promoter, exon 1, and intron 1 sequences that contain several TCF/LEF binding sites; d2EGFP (Jho et al., 2002)
    ins-TOPEGFP Six LEF/TCF consensus binding sites and a minimal thymidine kinase promoter derived from pTOPFLASH (Moriyama et al., 2007)
    LEF-EGFP Seven multimerized LEF-1 binding sites with a c-fos minimal promoter (current report)