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. 2012 Apr;2(4):681–694. doi: 10.1002/ece3.199

Table 1.

Proteins identified in this study.

Spot Protein name Access number1 MASCOT score2 Theoretical mass Theoretical pI Sequence coverage (%) Peptide number Day(s) Treatment(s) involved3 Spot volume4
1205 Ferritin heavy chain-like protein Q8T4R8_AEDAE 78 23,802 5.64 33 8 5 E, V -
157 Catalase Q1HRH7_AEDAE 92 48,836 7.28 27 10 5 V -
265 Catalase Q1HRH7_AEDAE 100 48,836 7.28 26 10 5 E, V -
438 Catalase Q1HRH7_AEDAE 88 48,836 7.28 24 9 5 V -
9 Actin Q17KG3_AEDAE 127 42,148.9 5.22 39 15 15 E, VE +
29 Actin Q17KG3_AEDAE 60 42,148.9 5.22 23 11 5 E, V -
74 Actin Q17KG3_AEDAE 53 42,148.9 5.22 21 8 5 V -
115 Actin Q178B0_AEDAE 67 41918.8 5.29 20 9 15 E, VE -
1205 Vacuolar ATPase B subunit Q9XYC8_AEDAE 96 55,466.3 5.38 27 10 5 E, V -
175 Enolase Q17KK5_AEDAE 51 46,877.1 6.28 21 9 5 and 15 E, V -
3241 Enolase Q17KK5_AEDAE 161 46,877.1 6.28 44 19 5 V -
3243 Enolase Q17KK5_AEDAE 104 46,877.1 6.28 30 10 5 V -
234 V-type proton ATPase catalytic subunit A VATA_AEDAE 52 68,527.6 5.26 11 6 5 E, V -
131 Glutathione S-transferase Q16P79_AEDAE 149 27,034 5.24 50 16 5 V -
135 Arginine or creatine kinase Q1HR67_AEDAE 152 40,191 5.97 38 14 5 and 15 VE +
2081 Arginine or creatine kinase Q1HR67_AEDAE 78 40,191 5.97 30 9 5 V -
35 Q17G18_AEDAE 165 23,677 4.71 46 13 5 E, V -
181 Q16YP3_AEDAE 84 29,644 4.89 35 8 5 E, V -
1

“Access number” refers to accession number in SwissProt and TrEMBL protein databases.

2

“MASCOT scores” > 50 indicate extensive homology while those > 68 indicate significant identity (P < 0.05).

3

V, V. culicis only; E, E. aedis only; VE, coinfection with V. culicis and E. aedis.

4

Spot volume relative to uninfected larvae on same day(s) of sampling; + = higher, − = lower.

5

Spot 120 was assigned two possible identities.