Table 1.
Proteins identified in this study.
| Spot | Protein name | Access number1 | MASCOT score2 | Theoretical mass | Theoretical pI | Sequence coverage (%) | Peptide number | Day(s) | Treatment(s) involved3 | Spot volume4 |
|---|---|---|---|---|---|---|---|---|---|---|
| 1205 | Ferritin heavy chain-like protein | Q8T4R8_AEDAE | 78 | 23,802 | 5.64 | 33 | 8 | 5 | E, V | - |
| 157 | Catalase | Q1HRH7_AEDAE | 92 | 48,836 | 7.28 | 27 | 10 | 5 | V | - |
| 265 | Catalase | Q1HRH7_AEDAE | 100 | 48,836 | 7.28 | 26 | 10 | 5 | E, V | - |
| 438 | Catalase | Q1HRH7_AEDAE | 88 | 48,836 | 7.28 | 24 | 9 | 5 | V | - |
| 9 | Actin | Q17KG3_AEDAE | 127 | 42,148.9 | 5.22 | 39 | 15 | 15 | E, VE | + |
| 29 | Actin | Q17KG3_AEDAE | 60 | 42,148.9 | 5.22 | 23 | 11 | 5 | E, V | - |
| 74 | Actin | Q17KG3_AEDAE | 53 | 42,148.9 | 5.22 | 21 | 8 | 5 | V | - |
| 115 | Actin | Q178B0_AEDAE | 67 | 41918.8 | 5.29 | 20 | 9 | 15 | E, VE | - |
| 1205 | Vacuolar ATPase B subunit | Q9XYC8_AEDAE | 96 | 55,466.3 | 5.38 | 27 | 10 | 5 | E, V | - |
| 175 | Enolase | Q17KK5_AEDAE | 51 | 46,877.1 | 6.28 | 21 | 9 | 5 and 15 | E, V | - |
| 3241 | Enolase | Q17KK5_AEDAE | 161 | 46,877.1 | 6.28 | 44 | 19 | 5 | V | - |
| 3243 | Enolase | Q17KK5_AEDAE | 104 | 46,877.1 | 6.28 | 30 | 10 | 5 | V | - |
| 234 | V-type proton ATPase catalytic subunit A | VATA_AEDAE | 52 | 68,527.6 | 5.26 | 11 | 6 | 5 | E, V | - |
| 131 | Glutathione S-transferase | Q16P79_AEDAE | 149 | 27,034 | 5.24 | 50 | 16 | 5 | V | - |
| 135 | Arginine or creatine kinase | Q1HR67_AEDAE | 152 | 40,191 | 5.97 | 38 | 14 | 5 and 15 | VE | + |
| 2081 | Arginine or creatine kinase | Q1HR67_AEDAE | 78 | 40,191 | 5.97 | 30 | 9 | 5 | V | - |
| 35 | — | Q17G18_AEDAE | 165 | 23,677 | 4.71 | 46 | 13 | 5 | E, V | - |
| 181 | — | Q16YP3_AEDAE | 84 | 29,644 | 4.89 | 35 | 8 | 5 | E, V | - |
“Access number” refers to accession number in SwissProt and TrEMBL protein databases.
“MASCOT scores” > 50 indicate extensive homology while those > 68 indicate significant identity (P < 0.05).
V, V. culicis only; E, E. aedis only; VE, coinfection with V. culicis and E. aedis.
Spot volume relative to uninfected larvae on same day(s) of sampling; + = higher, − = lower.
Spot 120 was assigned two possible identities.