Table 1.
Number of samples for adult mussels, three host fish species, juvenile mussels, and infested fish gills per site. Ethanol: samples stored in 99% ethanol for three months at 20°C before DNA extraction; fresh: DNA extraction performed immediately after sampling; kit: DNA extraction by Macherey-Nagel NucleoSpin® tissue kit; P-C: DNA extraction by phenol–chloroform extraction method.
| Species | Sampling site | Country | Type of water body | Drainage basin | Number of samples |
|---|---|---|---|---|---|
| Adult freshwater mussels | |||||
| Class Bivalvia | |||||
| Order Unionoida | |||||
| Family Unionidae | |||||
| Subfamily Unioninae | |||||
| Tribe Anodontini | |||||
| Anodonta anatina (Linnaeus 1758) | Arbi | Estonia | Lake | Narva | 4 |
| Kieferndorf | Germany | Fish pond | Rhine | 2 | |
| Unterpreuschwitz | Germany | Fish pond | Rhine | 2 | |
| Aquarium shop Duscher, Passau | Germany | - | - | 2 | |
| Ratzteich | Austria | Quarry pond | Danube | 2 | |
| Anodonta cygnea (Linnaeus 1758) | Arbi | Estonia | Lake | Narva | 2 |
| Quidenham Mere | UK | Lake | Great Ouse | 2 | |
| Wellsee | Germany | Lake | Schwentine | 2 | |
| Wellsee, outflow | Germany | Lake outflow | 2 | ||
| Unterpreuschwitz | Germany | Fish pond | Rhine | 2 | |
| Staffelsee | Germany | Lake | Danube | 2 | |
| Kieferndorf | Germany | Fish pond | Rhine | 2 | |
| Hornbach gardening and pet shop, Bamberg | Germany | - | - | 1 | |
| Ratzteich | Austria | Quarry pond | Danube | 2 | |
| Pseudanodonta complanata (Rossmässler 1835) | Schwentine | Germany | River | Schwentine | 4 |
| Danube | Austria | River | Danube | 1 | |
| Morava | Austria | River | Danube | 1 | |
| Sinanodonta woodiana (Lea 1834) | Hornbach gardening and pet shop, Bamberg | Germany | - | - | 3 |
| Hornbach gardening and pet shop, Passau | Germany | - | - | 3 | |
| Dehner gardening and pet shop, Nuernberg | Germany | - | - | 3 | |
| Pet shop Schmid, Traunstein | Germany | - | - | 2 | |
| Tribe Unionini | |||||
| Unio crassus (Philippson 1788) | Schwentine | Germany | River | Schwentine | 3 |
| Ischler Ache | Germany | River | Danube | 3 | |
| Donaumoosach | Germany | River | Danube | 3 | |
| Unio pictorum (Linnaeus 1758) | Thames | UK | River | Thames | 2 |
| Schwentine | Germany | River | Schwentine | 3 | |
| Ischler Ache | Germany | River | Danube | 3 | |
| Staffelsee | Germany | Lake | Danube | 1 | |
| Dehner gardening and pet shop, Nuernberg | Germany | - | - | 3 | |
| Unio tumidus (Philippson 1788) | Thames | UK | River | Thames | 2 |
| Schwentine | Germany | River | Schwentine | 6 | |
| Aquarium shop Duscher, Passau | Germany | - | - | 2 | |
| Family Margaritiferidae | |||||
| Margaritifera margaritifera (Linnaeus 1758) | Hasselån | Sweden | River | Ljungan | 2 |
| Kärmsjöbäcken | Sweden | River | Ångermanälven | 2 | |
| Mattarbodbäcken | Sweden | River | Gideälven | 2 | |
| Nister | Germany | River | Rhine | 2 | |
| Wolfsteiner Ohe | Germany | River | Danube | 3 | |
| Aitrach | Germany | River | Danube | 2 | |
| Host fish | |||||
| Class Osteichthyes | |||||
| Order Salmoniformes | |||||
| Salmo trutta (Linnaeus 1758) | Hatchery at River Mauka (stock from River Isar) | Germany | - | Danube | 4 |
| Order Cyprinodontiformes | |||||
| Leuciscus leuciscus (Linnaeus 1758) | Glonn | Germany | River | Danube | 2 |
| Order Gasterosteiformes | |||||
| Gasterosteus aculeatus (Linnaeus 1758) | Moosach (flood channel) | Germany | Flood channel | Danube | 2 |
| Juvenile Margaritifera margaritifera | |||||
| Fresh tissue/kit | Hatchery at TUM (population from River Regnitz) | Germany | - | Elbe | 1 |
| Fresh tissue/kit | 1 × 3 | ||||
| Fresh tissue/P-C | 1 | ||||
| Fresh tissue/P-C | 1 × 3 | ||||
| Salmo trutta gills infested by Margaritifera margaritifera glochidia | |||||
| Fresh tissue/kit | Hatchery at TUM (stock as above) | Germany | - | - | 8 ×∼100 |
| Fresh tissue/P-C | Germany | 8 ×∼100 | |||
| Ethanol tissue/kit | Germany | 8 ×∼100 | |||
| Ethanol tissue/P-C | Germany | 8 ×∼100 | |||