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Effect of 18α-GA on DNA binding activities of transcription factors by EMSA in human and rat HSCs. The activities of SP-1, AP-1 and NF-κB decreased gradually after 18α-GA treatment which was in a time-dependent manner. I, LX-2; II , CFSC. A, AP-1; B, SP-1; C, NF-κB. 1, negative control; 2, competing reaction of probe; 3, competing reaction of mutant probe; 4, Super-shift reaction; 5-9, sample's reaction; 5, 0 h; 6, 12 h; 7, 24 h; 8, 36 h; 9, 48 h.
