Table 2.
primers for target genes in CFSC
| Genes | Primers | Size (bp) |
|---|---|---|
| Smad2 | F: 5'- GATGGTCGTCTTCAGGTGTCTC -3' R: 5'- CCTCTGGTAGTGGTAAGGGTTC -3' |
174 |
| Smad3 | F: 5'-AAATGACAGCAGCAGGGACACTA -3' R: 5'-TGAGGAGGTAGGACCCACAGTAGA-3' |
176 |
| Smad4 | F: 5'- ATCACTATGAGCGGGTTGTC -3' R: 5'- GAATGTCCTTCCGTGGGTAA -3' |
142 |
| Smad7 | F: 5'- CTCGGAAGTCAAGAGGCTGTG -3' R: 5'- CCATCGGGTATCTGGAGTAAGGA -3' |
137 |
| TβRⅠ | F: 5'- CTGCCTGCTTCTCATCGTGT -3' R: 5'- AACTTTGTCTGTGGTCTCGG -3' |
154 |
| TβRⅡ | F: 5'- CCC TACTCTGTCTGTGGATGA -3' R: 5'- GACGTCATTTCCCAGAGTAC -3' |
152 |
| COL1A2 | F: 5'- TGACCAGCCTCGCTCACAG-3' R: 5'- 5 GCGGGCAGGGTTCTTTCTA -3' |
127 |
| COL3A1 | F: 5'- TCCCAGAACATTACATACCACT -3' R: 5'- GCTATTTCCTTCAGCCTTGA -3' |
126 |
| GAPDH | F: 5'- GATGAGATTGGCATGGCTTT -3' R: 5'- GAGAAGTGGGGTGGCTT -3' |
196 |