Skip to main content
. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: Eur Respir J. 2011 Feb 24;38(1):15–28. doi: 10.1183/09031936.00029210

Figure 4.

Figure 4

Identification of heritable GSTT1 copy number variation. A. Chromosome 22 location of the GSTT1 gene in relation to known CNV. The relative locations of two Affymetrix Human SNP 5.0 CNV probe sets and an Applied Biosystems TaqMan RT-PCR primer for GSTT1 copy number variation are also represented below. B. CNV genotypes by microarray. Normalized intensity levels from the study population (n = 70) of the two CNV probe sets indicated in panel A are plotted on the abscissa and the ordinate. Subjects homozygous null for the GSTT1 CNV (△), heterozygotes (▲) and homozygous wild type individuals (▲) are as shown. C. Correlation of PCR assessed GSTT1 CNV genotypes to microarray assessment. TaqMan RT-PCR assays for the GSTT1 CNV using the probe shown in panel A in a random subset of subjects (n = 31) of microarray-defined GSTT1 genotype [homozygous nulls, heterozygotes (null/+) and diploid subjects (+/+)] are represented on the abscissa. The reference haploid subject (“Ref”) is shown on the left, with copy number calls by RT-PCR on the ordinate. Null/null individuals had a RT-PCR product below the detection limit as indicated. Duplicate measurements are shown for each subject, linked by dashed lines.