Table I.

Demographics of the Study Population and Biologic Samples¹

	Small airway epithelium		Alveolar macrophages
Parameter	Healthy nonsmokers	Healthy smokers	Healthy nonsmokers	Healthy Smokers
n	35	35	22	34
Sex (male/female)	28/7	22/13	17/5	24/10
Age (yr)	43 ± 11	43 ± 6	41 ± 8	43 ± 7
Race (B/W/O)2	17/14/4	21/8/6	13/6/3	20/9/5
Smoking history (pack-yr)	0	24 ± 13	0	26 ± 17
Urine nicotine (ng/ml)	0	1029 ± 1045	0	796 ± 878
Urine cotinine (ng/ml)	0	1158 ± 861	0	1060 ± 717
Blood       carboxyhemoglobin (%)	0.6 ± 0.9	2.2 ± 2.0	0.6 ± 0.8	2.4 ± 2.4
Pulmonary function parameters3
FVC	108 ± 12	109 ± 12	106 ± 11	109 ± 11
FEV1	108 ± 14	109 ± 13	106 ± 11	109 ± 12
FEV1/FVC	82 ± 7	82 ± 4	82 ± 5	82 ± 4
TLC	102 ± 13	101 ± 12	96 ± 8	99 ± 12
DLCO	98 ± 15	97 ± 12	95 ± 9	98 ± 13
Epithelial cells
Number recovered	6.0 × 106	6.1 × 106
% epithelial cells	99.5 ± 0.9	99.7 ± 0.6
% inflammatory cells	0.5 ± 0.9	0.3 ± 0.6
% ciliated	74.4 ± 7.2	71.4 ± 7.4
% secretory	7.0 ± 3.7	7.4 ± 3.1
% basal	10.7 ± 4.7	10.1 ± 3.4
% undifferentiated	7.3 ± 3.2	10.8 ± 5.7
BAL4 cells
Number recovered x106			14.1 ± 9.1	31.2 ± 20.3
% viability			96.6 ± 1.2	96.3 ± 1.1
% alveolar macrophages5			96.0 ± 1.3	95.7 ± 1.7
% lymphocytes			2.7 ± 1.6	2.8 ± 1.7
% polymorphonuclear cells			0.9 ± 1.1	1.1 ± 1.3
% epithelial cells			0.4 ± 0.7	0.4 ± 0.4

¹Data is presented as mean ± standard deviation.

²B = black, W = white, O = other.

³Pulmonary function testing parameters are given as percent of predicted value with the exception of FEV1/FVC, which is reported as % observed; FVC - forced vital capacity, FEV1 - forced expiratoryvolume in 1 sec, TLC - total lung capacity, DLCO - diffusing capacity of the lung for carbon monoxide.

⁴Bronchoalveolar lavage.

⁵Listed is the % AM before purification; after purification the AM were >98% pure.