Figure 8.

Bafilomycin A₁ and YM201636 treatments increased the number of GFP-WIPI-1 positive autophagosome-like vesicles entrapping Staphylococci. GFP-WIPI-1 U2OS cells were infected with S. aureus USA300 in DMEM/FCS in the absence (control) or presence of 200 nM bafilomycin A₁ (Baf A₁), 800 nM YM201636 (YM), or with both (Baf A₁/YM) for 2 h. Images were automatically acquired (not shown). The number of GFP-WIPI-1 puncta-positive cells ((a), right panel) and the number of GFP-WIPI-1 puncta per cell ((b), right panel) was determined. From 100 infected cells for each of the treatment condition, the number of cells displaying GFP-WIPI-1 positive autophagosomal-like vesicles entrapping S. aureus USA300 ((a), left panel) and the number of GFP-WIPI-1 autophagosomal-like vesicles entrapping S. aureus USA300 per cell ((b), left panel) was determined (n = 3). *P < 0.05, **P < 0.01, ns: not significant.