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Titration of rabbit serum. One μg/mL 89.6gp140 linker protein or 1 μg/mL gp41Fc was coated on microwell plates. After blocking the wells, 2-fold serially diluted rabbit serum was added to the plates. Bound Abs were detected using HRP-anti-rabbit IgG, F(ab’)2 and ABTS as a substrate.