Table 1.

Neutralization activity (IC₅₀) of rabbit IgGs FT1214 and FT1215 against a panel of primary isolates of HIV-1 and HIV-2 in a PBMC assay. AZT was used as a positive control with 100% inhibition at a concentration equal to 0.1μM, where the measured cell toxicity was undetectable (IC₅₀ = 0.002μM, TC₅₀ >1μM). TC₅₀s of both FT1214 and FT1215 IgGs were over 50μg/mL for all isolates tested. IC₅₀s were converted to the serum dilutions based on the amount of FT1214, 1215 IgG isolated from the corresponding serum, 6.7mg and 6.0mg per mL serum, respectively.

Virus	Subtype	Coreceptor	FT1214 IgG IC₅₀ (μg/mL)	FT1215 IgG IC₅₀ (μg/mL)	AZT IC₅₀ (nM)	FT1214 serum dilution	FT1215 serum dilution
92UG037	A	R5	43.8	>50.0	0.55	1:152	<1:120
92UG029	A	X4	>50.0	>50.0	8.5	<1:133	<1:120
92BR014	B	R5X4	>50.0	>50.0	<0.10	<1:133	<1:120
92TH026	B	R5	3.31	>50.0	0.86	1:2,015	<1:120
Ba-L	B	R5	22.2	>50.0	3.67	1:300	<1:120
92BR025	C	R5	8.91	>50.0	1.12	1:749	<1:120
93IN101	C	R5	22.7	>50.0	3.92	1:294	<1:120
92UG001	D	R5X4	>50.0	>50.0	1.67	<1:133	<1:120
UG92024	D	X4	35.3	>50.0	5.63	1:189	<1:120
92UG046	D	X4	>50.0	>50.0	2.35	<1:133	<1:120
CMU08	E	X4	>50.0	>50.0	0.79	<1:133	<1:120
93BR019	B/F	X4	>50.0	>50.0	2	<1:133	<1:120
93BR020	F	R5X4	>50.0	>50.0	1.42	<1:133	<1:120
93BR029	B/F	R5	9.93	>50.0	1.12	1:672	<1:120
RU132	G	R5	>50.0	>50.0	3.31	<1:133	<1:120
G3	G	R5	1.75	>50.0	0.54	1:3,811	<1:120
BCF01	Group O	R5	>50.0	>50.0	1.59	<1:133	<1:120
BCF02	Group O	R5	12.4	>50.0	1.98	1:538	<1:120
BCF03	Group O	R5	>50.0	>50.0	2.92	<1:133	<1:120
CDC310319	HIV-2		45	>50.0	1.54	1:148	<1:120
CDC310342	HIV-2		1.8	2.84	0.21	1:3,706	1:2,113
Percentage of virus neutralized			52	5	100