Skip to main content
. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: Wound Repair Regen. 2011 Apr 21;19(3):411–419. doi: 10.1111/j.1524-475X.2011.00688.x

Figure 2. Glyc101 induced TNFα production and gene expression in macrophages isolated from murine wound.

Figure 2

A. Murine wound macrophages were treated with Glyc101 at varying dosages of 5–100 μg/ml for 24h; B. Kinetics of TNFα production by wound macrophages in culture media was measured following treatment with Glyc101 (20 μg/ml) for 0–24 h; C. TNFα mRNA expression was measured in wound macrophages treated with Glyc101 (20 μg/ml) for 0–6 h using real-time PCR. TNFα mRNA level in cells was normalized against GAPDH (housekeeping gene); D. Murine wound macrophages were treated with Glyc101 for 24 h at the indicated dosages. The % loss in viability of macrophage following GLyc101 treatment was determined using lactate dehydrogenase (LDH) leakage assay. Data are mean ± SD (n=3). *, p<0.05; **, p<0.001 compared to control cells.