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. Author manuscript; available in PMC: 2012 Jul 18.

Published in final edited form as: Apoptosis. 2010 Jul;15(7):834–849. doi: 10.1007/s10495-010-0494-8

Fig. 4 — Apoptosis was induced in Jurkat cells by addition FasL for 4h. Cells were loaded with Fluo-3 AM 30min prior to flow cytometry analysis. Intracellular Ca²⁺ content was analyzed in either Fluo-3 fluorescence versus forward scatter dot plots (A) or Fluo-3 fluorescence histograms (B). Shown are either a single representative experiment from three independent experiments, (A and B), or averaged values ± SEM from three independent experiments (C). Asterisks (*) indicate statistical significance (P<0.05) between the two experimental conditions at each concentration of FasL.

Fig. 4 — Apoptosis was induced in Jurkat cells by addition FasL for 4h. Cells were loaded with Fluo-3 AM 30min prior to flow cytometry analysis. Intracellular Ca²⁺ content was analyzed in either Fluo-3 fluorescence versus forward scatter dot plots (A) or Fluo-3 fluorescence histograms (B). Shown are either a single representative experiment from three independent experiments, (A and B), or averaged values ± SEM from three independent experiments (C). Asterisks (*) indicate statistical significance (P<0.05) between the two experimental conditions at each concentration of FasL.