Table I.
Concentrations of protein carbonyls (nmol mg−1 protein) in cell extracts of yeast treated with different menadione concentrations (5–100 mM) in phosphate or bicarbonate buffers. Data shown are the mean ± SEM (n = 4).
| Buffer | Menadione (mM)
|
|||||
|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 25 | 50 | 100 | |
| 25 mM | ||||||
| Phosphate | 6.2 ± 0.61 | 5.0 ± 0.77 | 4.4 ± 0.29* | 3.4 ± 0.27* | 3.7 ± 0.43* | 3.5 ± 0.28* |
| Bicarbonate | 4.3 ± 0.58 | 4.0 ± 0.21 | 4.2 ± 0.58 | 4.9 ± 0.64 | 3.9± 0.48 | 4.4 ± 0.43 |
|
| ||||||
| 50 mM | ||||||
| Phosphate | 2.7 ± 0.21 | 3.3 ± 0.36 | 2.9 ± 0.36 | 2.5 ± 0.35 | 3.4 ± 0.68 | 3.0 ± 0.29 |
| Bicarbonate | 2.8 ± 0.26 | 3.6 ± 0.68 | 3.0 ± 0.46 | 3.8 ± 0.32 | 3.3 ± 0.15 | 3.4 ± 0.29 |
*
Significantly different from respective controls (untreated cells), P<0.05.