Figure 3. Quantitative real-time PCR analysis of DE genes in elk brain tissues (A) and elk spleen, RPLN and tonsil tissues (B) in two different animals. DE genes associated with neuronal signaling include: α-Synuclein A (SNCA); Neuropeptide Y (NPY), Cocaine amphetamine responsive transcript (CART); Synaptosomal-associated protein 25KD (SNAP25), Tachykinin (TAC1); calcium ion regulation: Parvalbumin (PVALB), Visinin-like (VNSL1) and Angotensin II receptor (AGTRL); apoptosis: Human T-cell leukemia virus type I (Tax1BP1) and Superoxide dismutase (SOD1); immune and inflammatory response: Chemokine 20 (CCL20) and Serum amyloid A-like (SAA). Gene expression was compared between control and PRPCWD infected elk.