Table 1. Gene Ontology (GO) analysis of differentially expressed genes with ≥ 2.0 fold change, associated with CWD disease in brain tissues of elk.
| GO Function (Biological and Molecular processes) | Gene ID | Gene Name | BR | MB | TH |
|---|---|---|---|---|---|
| Apoptosis |
Bt.49637 Bt.17392 Bt.49336 Bt.49674 Bt.5427 Bt.87330 |
Superoxide dismutase 1 Tax1 binding protein 1 Ras homolog gene family Voltage-dependent anion channel 1 Secretogranin II Scinderin |
-3.96 -2.26 -3.44 2.46 |
-2.28 |
-2.01 -2.21 -2.70 |
| Calcium binding and regulation |
Bt.61440 Bt.61911 Bt.65801 Bt.49702 |
Parvalbumin Multiple coagulation factor deficiency 2 Neurocalcin delta Visinin-like |
-2.07 3.24 -2.31 |
|
-3.77 -2.79 |
|
Nervous system function and synaptic transmission |
Bt.12770 Bt.12930 Bt.20015 Bt.22135 Bt.30532 Bt.35938 Bt.40062 Bt.5457 Bt.67878 Bt.89014 Bt.42529 |
Synuclein, α Tachykinin, precursor 1 Cocaine and amphetamine responsive transcript Neurensin 1 Cholecystokinin Calcyon neuron-specific vesicular protein Amyloid β (A4) precursor protein Growth associated protein 43 Neuron specific gene family member 1 Neurofilament, medium polypeptide Synaptosomal-associated protein, 25kDa |
-2.32 -2.27 -2.40 -3.74 -2.16 |
-2.49 -3.36 |
-3.30 -3.05 -2.01 -3.69 -2.22 -2.01 -2.88 -2.78 -2.50 -4.17 |
|
Cytoskeleton organization |
Bt.49700 Bt.13391 Bt.49547 Bt.53255 Bt.6630 |
Allograft inflammatory factor 1 Capping protein muscle Z-line Lymphocyte cytosolic protein 1 Thy-1 cell surface antigen Calponin 1, basic, smooth muscle |
2.03 -2.19 2.29 |
2.16 |
-2.36 |
|
Binding |
Bt.13833 Bt.20597 Bt.22187 Bt.24896 Bt.34711 Bt.35816 Bt.37226 Bt.42457 Bt.42866 Bt.43398 Bt.49619 Bt.49713 Bt.5448 Bt.60794 Bt.62630 Bt.64032 Bt.6645 Bt.6830 Bt.74143 Bt.76429 Bt.76948 Bt.87082 Bt.91195 Bt.207 Bt.34807 |
RING1 and YY1 binding protein Tetratricopeptide repeat domain 35 Regulator of chromosome condensation (RCC1) and BTB (POZ) domain Kinesin-associated protein 3 Leucine rich repeat transmembrane neuronal 1 Neurotrimin Fibroblast growth factor 12 Hypothetical protein LOC506058 Metallothionein 1E TRIM6-TRIM34 Spastic paraplegia 3A Creatine kinase, mitochondrial 1 Chromogranin B Alpha-2-macroglobulin Mal, T-cell differentiation protein 2 Lymphocyte antigen 75 RNA-binding region (RNP1, RRM) containing 3 Septin 7 Ermin, ERM-like protein Ceruloplasmin (ferroxidase) FK506 binding protein 1B, 12.6 kDa GTP-binding protein 8 (putative) Succinate-CoA ligase, ADP-forming, Transthyretin (prealbumin, amyloidosis) Neurofilament, heavy polypeptide 200kDa |
-2.37 -2.40 -2.12 -2.22 2.19 7.86 2.01 -2.17 -2.88 -2.41 4.14 -4.36 -2.24 |
-2.07 3.97 2.23 -2.91 -2.26 2.42 -2.02 |
-2.00 -2.48 -2.65 -2.63 4.89 -2.27 -2.81 -2.22 2.93 2.04 -2.44 -2.19 -2.28 -4.52 |
|
Immune and inflammatory response |
Bt.1035 Bt.5356 Bt.611 Bt.64791 Bt.73288 Bt.8552 Bt.88538 |
Fc fragment of IgG, receptor Similar to MHC class II antigen Chemokine (C-X-C motif) ligand 2 Acyloxyacyl hydrolase (neutrophil) Complement component 4A Major histocompatibility II, DR α Major histocompatibility II, DRB3 |
3.22 2.31 2.54 |
2.01 |
2.02 2.96 -2.03 2.15 2.56 |
|
Post-translational protein modification and protein folding |
Bt.26656 Bt.36328 Bt.39123 Bt.73670 Bt.22615 Bt.53009 |
Mitogen-activated protein kinase 10 P21 (CDKN1A)-activated kinase 3 Protein tyrosine phosphatase Carboxypeptidase E Protein-L-isoaspartate O-methyltransferase Chaperonin containing TCP1 |
-2.57 |
-2.69 -2.52 |
-2.32 -2.04 -2.43 -2.12 |
|
Proteolysis |
Bt.12553 Bt.52393 Bt.58848 |
Haptoglobin Cathepsin H Ubiquitin carboxyl-terminal esterase L1 |
2.14 |
|
2.33 -3.05 |
|
Regulation of biological process |
Bt.49566 Bt.35977 Bt.4804 Bt.67202 Bt.63542 Bt.25016 Bt.25332 Bt.64131 Bt.52808 Bt.64620 Bt.4832 Bt.49563 Bt.4185 Bt.48705 Bt.48894 |
SH3 domain binding glutamic acid-rich protein like 3 Radixin Cyclin-dependent kinase inhibitor 1C Protein tyrosine phosphatase-like A Similar to Chain D, Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor In Complex With Calmodulin Similar to EBF1 protein Nuclear receptor subfamily 4 RAB2A, member RAS oncogene family TAF9 RNA polymerase II Polymerase (RNA) II polypeptide A Ribosomal protein S15 RNA binding motif protein 3 NHP2 non-histone chromosome protein 2-like 1 GINS complex subunit 4 Protein S (α) |
2.12 -2.00 -2.52 -2.31 -2.50 -2.15 -2.31 2.22 -2.08 -2.03 2.53 |
|
-2.42 -2.17 2.10 |
|
Signal transduction |
Bt.3704 Bt.53744 Bt.53955 Bt.5546 Bt.62010 Bt.64619 |
Stathmin-like 2 Membrane-spanning 4-domains Chimerin (chimaerin) 1 Guanine nucleotide binding protein Syndecan binding protein GNAS complex locus |
-2.50 -2.13 -2.14 -4.00 |
-2.46 -2.09 |
-2.77 -2.44 |
|
Transport |
Bt.2235 Bt.26950 Bt.4482 Bt.46431 Bt.46903 Bt.48572 Bt.49570 Bt.52307 Bt.61189 Bt.62049 Bt.87586 Bt.88575 Bt.14573 |
GDP dissociation inhibitor 1 Solute carrier family 39 Macrophage scavenger receptor 1 Kv channel interacting protein 4 Potassium inwardly-rectifying channel Guanine nucleotide binding protein ATPase, Na+/K+ transporting ATPase, H+ transporting, lysosomal 42kDa ATP synthase, H+ transporting, mitochondrial F0 complex Sodium channel, voltage-gated Similar to Kinesin-like protein KIF3A Tubulin, β 2B ELMO/CED-12 domain containing 1 |
2.60 2.01 -2.35 |
-2.16 3.40 2.26 |
-2.05 5.16 -2.40 -2.30 -2.79 -2.09 -2.03 -2.61 -3.24 |
|
Metabolic process |
Bt.97115 Bt.20383 Bt.60921 Bt.87389 |
Stearoyl-CoA desaturase Microsomal glutathione S-transferase 1 Citrate synthase Glyceraldehyde-3-phosphate dehydrogenase |
-2.69 -2.41 |
-2.11 |
-2.15 |
|
Glycolysis |
Bt.15319 Bt.5345 |
Phosphoglycerate mutase 1 (brain) Malate dehydrogenase 1, NAD |
|
-2.36 |
-2.15 -2.48 |
|
Cell adhesion |
Bt.9656 Bt.9807 |
Collagen, type XI, α 1 Glycoprotein (transmembrane) nmb |
-2.36 2.71 |
|
|
|
Hydrolase activity |
Bt.49237 |
Transmembrane protein 55ª |
|
|
-2.04 |
BR, brainstem; MB, midbrain; TH, thalamus.